Agricultural Scientists Recruitment Board NET SYLLABUS
| Unit 1: General Bacteriology |
Milestones in the development of microbiology, Classification and nomenclature of bacteria. Structure, function and chemistry of bacterial nuclear apparatus. Cytoplasm, Intracellular granules, Cell wall, Cytoplasmic membrane, Mesosomes, Cap sale, Flagella, Fimbriae, Endospore, Protoplasts, Spheroplasts , L-forms , involution forms . Bacterial stains, staining and microscopy. Growth and nutritional requirements of bacteria. Bacterial enzymes. Respiration in bacteria. Carbohydrate protein, fat and nucleic acid metabolism in bacteria. Reproduction and growth phase of bacteria. Effects of chemical and physical agents and antibiotics. Bacterial variations including transduction, transformation and conjugation. Bacterial vaccines and toxins.
The role of microbial toxins in the pathogenesis of diseases; Biochemical and biological characteristics of toxins produced by various bacteria. Toxin produces Gram positive and Gram-negative bacteria. Properties and clinical conditions produced by different bacterial toxins.
| Unit 2: Systematic Bacteriology |
Systematic study of bacteria belonging to genera Borrelia, Leptospira, Campylobacter, Pseudomonas, Brucella, Bordetella, Escherichia, Citrobacter, Salmonella, Shigella, Klebsiella, Enterobacter, Proteus, Vibrio, Haemophilu s , Staphylococcus, Streptococcus, Bacillus, Clostridium, Listeria, Erysipelothrix, Corynebacterium, nocardia, Rickettsia, Chlamydia, Mycoplasma, Acholeplasma, Spiroplasma, Anaeroplasma and Thermopl as ma, Rhodococcus, Mycobacterium and Neisseria.
| Unit 3: General Virology |
Historical development of virology. Evolution, classification and nomenclature of viruses. Biophysical and biochemical characteristics of viruses. Cultivation of viruses and their growth in cell culture, embryonated eggs and experimental animals. Purification and concentration of viruses s. Qualitative and quantitative assay of viruses. Viral replication, Virus-host cell relationships, Replication strategies of animal viruses and molecular pathogenesis for selected viral systems. La cut, persistent and chronic viral infections. Study of genetic variability of animal viruses through use of monoclonal antibodies, autoimmunity, immunosuppression and viral mutation in persistence infections. General principles of laboratory diagnosis of viral diseases. Epidemiology and pathology of viral infections. Immune mechanism v in viral diseases. Interference and interferon. Viral vaccines, point of action of antiviral molecules during the replication cycle of a virus and search for new antiviral compounds, viruses and gene therapy. Chemotherapy of viral infections.
| Unit 4: Systematic Virology |
Systematic study of RNA and DNA viruses in livestock and poultry with reference to antigenicity, cultivation, pathogenesis, epidemiology, diagnosis and immunity, RNA viruses: Retroviruses and lymphotropic viruses, Visna and maedi, Arboviruses, Rotaviruses . Birnaviruses. Picornaviruses. Bunyaviruses . Coronaviruses. Togaviruses , Paramyxoviruses , Orthomyxoviruses, Rhabdoviruses. Picornaviruses. Btinyavirus es. Arenaviruses. Arterial Viruses. Caliciviruses . Filoviruses , DNA viruses: Poxviruses . Hepadnaviruses. Iridoviruses , Adenoviruses, PapovaviruS( S. ParvoviruseS . Hepadnaviruses. Circoviruses. Unclassified viruses. Slow viruses Scrapie.
| Unit 5: Mycology |
General characteristics of fungi. Classification and study of pathogenic fungi- Epidermophyton,Microsporum,Trichophyton,Cryptococcus,Aspergillus,Blastomyces,Coccidioides,Histoplasma, Candida, Rhinosporidium,Contaminating fungi, Rhizopus, Mucor and Penicillium. Fungi causing mast Otis , abortion and mycotoxicosis .
| Unit 6: Immunology |
Historical Perspectives. Host-parasite relationships. Antigens . ypes of antigens. Properties and specificity of antigens. Factor determining antigenicity. Haptens and carriers. Heterophile antigens. Adjuvants . Mechanisms of action classification and their uses. Immunoglobulins — their classes and subclasses , stru :ture and function. Allotype s. Idiotypes. Genes coding for Igs. Generation of diversity. Monoclonal antibodies. Purification of antibodies. Theories of antibody formation. Lymphoid organs: primary, secondary and circulation of lymphocytes , ce 1s involved in the
immune response — lymphocytes, T lymphocytes, subsets and riatore of receptors.
Macrophages, Dendritic reticular cells, Langerhans cells . Cellular interactions Cell- mediated immune responses. Mechanism of interaction of antigen and antibody. The complement system. Classical and alternative pathways. Serological reactions: agglutination, precipitation, neutralization, CFT, FAT, ELIS/ , DII, RIA, etc, immunological methods as an aid to diagnosis, blotting techniques like Northern, Wcs terri blotting, Major, Histocompatibility complex: organization.
Nature of antigens and MHC restriction. Hypersensitivity immediate and delayed types, and mechanism of hypersensitivity. Mechanism of immunity, autoimmunity and immunological tolerance .
| Unit 7: Molecular Cell Biology (Vaccine 8s Diagnostic Technology) |
Role of biotechnology in diagnostics and vaccines, RNA electropherotyping. Probes – preparation of cDNA. Use of DNA probe in animal diseases diagnosis . Monoclonal antibodies. Application on monoclonal antibodies for diagnosis of animal diseases. Preparation of monoclonal antibodies. Nucleic acid hybridization Modern trends in vaccines. Recombinant DNA vaccines and their probable use in animal diseases. Bioinformatic tools in microbial research, Biosafety, Biosecurity, GJ4P and GLP.